Abstract: Garcinia mangostana L pericarp extract is known has active substances called

Xanthones which has strong antioxidant effects. This substance also has anti-inflammatory

and immunomodulation effects. However, the intercellular infection of Salmonella stimulates

macrophage to produce interleukin IL12 for enhancement of IFN-γ secretion. lFN γ, which

plays role for activates macrophage, acts as positive feedback in stimulating macrophages to

kill S. Typhimurium. Macrophages play role in the phagolysosome fusion process are

produced. This study aims to determine the effect of Crude Ethanolic Mangosteen Extract

(CEME) on IFN-γ and IL-12 secretion in mice infected with S. Typhimurium. This study

used 25 mice Balb/c. Mice divided into 5 groups consist of 5 mice in each group, including

positive control (mice were infected with S. Typhimurium), T0 (control with 40 mg/ml of

crude extract mangosteen pericarp without S. Typhimurium), T1, T2 and T3 (treatment with

20 mg/ml, 40 mg/ml and 60 mg/ml of crude extract mangosteen pericarp). Then, the mice

were necropsied and dissected to take the blood directly from the heart. Levels of IFN-γ and

IL-12 were analyzed using ELISA. Data were analyzed by One-Way ANOVA followed by

Post-Hoc test (LSD) to assess the comparison between groups. Results showed that CEME

increase serum IFN-γ and IL12 without infection. CEME increase serum IL12 in T1, T2and

T3 with a p-value 0,000. Other results showed decreases serum IFN-γ in T2 and T3 with a p￾value 0,000. CEME improve immune response through the increases serum IL-12 and

decreases serum IFN-γ in mice infected with S. Typhimurium.

Keywords : Extract, Garcinia mangostana, IFN- γ, IL-12, S. Typhimurium.

• Cereal and its products can be contaminated with fungi in the field, during drying, processing, transportation and subsequent storage, which may lead to secretion of mycotoxins under favourable condition. The aim of this study was the isolation and identification of some fungi associated with four kinds of Libyan food products of different trademarks. Twenty four (24) samples of couscous, macaroni, wheat flour and rice regularly used for human consumption by Libyan family were collected from local markets in the city of Alzawia, west of Tripoli, Libya. The results reveal isolation of 113 isolates belonging to nine genera: Penicillium, Aspergillus, Fusarium, Paecilomyces, Alternaria, Rhizopus, Mucor, Scopulariopsis and Cladosporum. Approximately 24 species were identified to belong to those isolated genera, several of which are known as main producer of mycotoxins especially A. flavus which are known to produce aflatoxins, Aspergillus niger, Aspergillus carbonarious, Penicillium chrysogenum and Penicillium verrucosum known to produce ochratoxin and Fusarium oxysporum and Fusarium chlamydosporum known to produce fumonisins and trichothecenes. Certainly, the occurrence of such types of mycotoxins can pose a health threatening risk for the consumer of those food items. Presence of these fungi in food products could be due to lack of good agriculture and food manufacturing practices throughout the food chain.
• Key words: Couscous, macaroni, wheat flour, rice, fungi, Libya.

Abstract

Aims: In the present work, the Libyan wild-growing Thymus capitatus essential oil (EO) was evaluated for its biological properties.

Methods and results: Carvacrol (68.19%) and thymol (12.29%) were found to be the main compounds of the oil. Antioxidant properties, determined by 2,2-diphenylpicrylhydrazyl (DPPH) assay, revealed that IC50 values were 119, 403 and 105 μg ml(-1) for oil, thymol and carvacrol respectively. Microdilution method showed strong antibacterial and especially antifungal potential. Tetrazolium (MTT) colorimetric assay indicated moderate cytotoxicity towards human cell lines MRC-5, HCT 116 and HT-29 (IC50 = 30-150 μg ml(-1)). In adhesion-inhibition assay oil and main compounds reduced adhesion of Escherichia coli and Listeria monocytogenes on colon cells HT-29 (51 and 39% of inhibition against L. monocytogenes and E. coli respectively).

Conclusions: Essential oil of Th. capitatus showed moderate cytotoxic activity, together with excellent antimicrobial effect, in particular against fungi, and significant potential to reduce pathogen colonization in colon.

Since the gender diversity of boards and reporting of earnings are two most debated issues in the corporate world, the paper examined how the presence of women directors on the corporate board influence earnings management practices. We found that firms with a higher number of female and independent female directors are adopting restrained earnings management practices in the UK. We further made a distinction between complex (high debt) and simple (low debt) companies, and the outcomes reveal that female directors have a positive effect on the earnings management in simple companies. The paper contributes to the debate on gender diversity on boards, and its impact on the use of accounting discretion in financial reporting.

Bioinformatics is a multi-disciplinary science focusing on the applications of computational methods and mathematical statistics to molecular biology. Choosing bioinformatics as specialization gives an opportunity to get involved with the most interesting computational techniques dealing with biological data to contribute to cure and diagnose some of genetic disorders that affect biological machines. The purpose of this library (which defines namespace BioQt), is to provide a set of routines for handling biological sequence data for Qt/C++ users (the full source code available on https://github.com/alrawab/BioQt). This thesis will shed the light on some modules of BioQt SDK such as exact string matching problem, Microsatellite Repeats, Palindromic sequences and sequence alignment algorithms (Longest Common Subsequence, Needleman-Wunsch and Smith-Waterman). This thesis examines and evaluates these challenging problems in bioinformatics by using Qt/C++.

Objective: We qualitatively evaluated the differences among susceptibility-weighted (SWI), magnitude (MAG), and high pass filtered phase (PHA) images in depicting interlobar differences in the appearance of the signal of the corticomedullary junction (CMJ). We conducted quantitative evaluation to validate the qualitative results.

Materials and Methods: We obtained SWI images from 25 preoperative brain tumor patients (12 men, 13 women, aged 19 to 82 years, mean, 52 years). Two trained neuroradiologists evaluated MAG, PHA, and SWI images. Qualitative evaluation of the CMJ signal and quantitative calculation of the relative signal ratio (RSR) percentages between the CMJ and deep white matter (WM) were conducted at 3 different slice levels of the brain independently for 4 different lobes (frontal, parietal, temporal, and occipital) and compared among MAG, PHA, and SWI. The extent of the area of the CMJ signal was graded on a 4-point scale (Grade 3, >75%; Grade 2, 50 to 75%; Grade 1, 25 to 50%; Grade 0, <25%). Data were statistically analyzed using a nonparametric Friedman test.

Results: The Kappa coefficients between the qualitative and quantitative grades were 0.002 for MAG, 0.0047 for PHA, and 0.050 for SWI. Qualitatively, on the PHA images and SWI, grades of the occipital lobes were significantly higher than those of the other lobes (P < 0.005). Quantitatively, PHA images showed statistically significant interlobar differences in RSR percentage values of the CMJ (P = 0.025).

Conclusion: Qualitatively, the appearance of the CMJ differed significantly among the different lobes of the brain on SWI and underlying PHA images but not on MAG images. Quantitatively, only PHA images showed significant interlobar differences in the RSR. PHA images are most sensitive to the CMJ signal contrast due to local paramagnetic iron content.