Lithostratigraphically, the Sidi As sid Formation (Upper Cretaceous) in the El Zintan section (JabalNafusah, NW Libya) consists of three units: upper marl, middle marl with intercalations of dolostone, and lower dolostone. Based on crystal size and shape, three types of dolomite have been classified. Fine crystalline dolomite (D1) consists of nonplanar dolomites, fine to medium crystalline dolomite (D2), and very coarse, consisting of mostly planar dolomites (D-3). The Cathodoluminescence technique also showed that most of these dolomites have bright yellow to red and dull orange luminescence and zoning. Based on X-ray diffraction (XRD), all dolomites are relatively well ordered and non to nearly stoichiometric dolomite. The stable isotopic studies and element analysis show that the major elements such as sodium (D1: 412.166 ppm; D2: 175 ppm; D3: 420 ppm), strontium (D1: 107 ppm; D2: 85 ppm; D3: 81.2 ppm), manganese (D1: 271 ppm; D2: 91 ppm; V3: 242 ppm), and iron (D1: 4856.66 ppm; D2: 373 ppm; D3: 3287 ppm) and the values of the stable isotope of oxygen (D1:-2.202‰; D2:-2.131; D3:-4.359‰) and carbon (D1: 2.080; D2: 2.076‰; D3: 1.581‰). The values of major elements and depletion of the carbon and oxygen isotopes can be related to the effects of temperature diagenetic, and mesosaline reflux of dolomitizing fluids during the early diagenesis. These dolomites originated in a tidal environment in this Formation. The major sources of magnesium for the dolomites are seawater and hypersaline fluids.

Exact string-matching algorithms have become very supreme in many bioinformatics tools. Despite the abundance and diversity of such algorithms, exposing them to real-time experimental analysis has been critical. This study was conducted to evaluate the efficiency of ten exact-string matching algorithms on large-scale genomic sequences from a runtime perspective. To define the most efficient algorithms are qualified to handle the short alphabet used for nucleic acid coding. The methodology promoted for this study was the factorial experiment with Randomized Complete Block Design (FRCBD). Under influence of four independent parameters, four levels of pattern lengths, four levels of pattern indices, two levels of programming languages, and ten levels of algorithmic architecture. The yield of the tested algorithms was calculated in nanoseconds. One-way ANOVA and Two-way ANOVA tests with post-hoc Games-Howell test were used separately for statistical analysis. In this study two widely accepted programming languages, C# and JAVA were used to speculate the possible effect of programing language on algorithm performance.

One of the unique combinations in the mammalian genome, that revolutionized concepts in the fields of genetics and molecular

pathology is what is termed the CpG islands. However, the accurate and rapid determination of CpG islands for DNA sequences remains

experimentally and computationally challenging. The main goal of this project is to design an offline, cross-platform CpG islands detection

tool. The Algorithm implemented in this study was the traditional sliding window algorithm by using the C++ programming language.

Three datasets were used for evaluating the performance of the application. The ANK1 gene, SPTB gene, and RET gene sequence files

were obtained from NCBI. In this study, the highest CGIs were reported in ANK1 (ankyrin 1) Gene which scored 13 successive islands

whereas the lowest score was reported in RET (ret proto-oncogene) Gene which shows only 6 islands. Generally, the program fulfills the

boundary limits as expected. We strongly recommend for further work, the implementation of other algorithms in addition to the sliding

window algorithm such as the Hidden Markov Model (HMM).

Abstract

The exposure of the developing foetal heart to hyperglycaemia in mothers with diabetes mellitus is a major risk factor for foetal cardiac complications that lead to heart failure. We studied the effects of hyperglycaemia on the layout of cardiac myofilament proteins in stem cell-derived cardiomyocytes and their possible underlying mechanisms. Mouse embryonic stem cells (mESCs) were differentiated into cardiac-like cells and cultured in media containing baseline- or high glucose concentrations. Cellular biomarkers were detected using Western blot analysis, immunocytochemistry, 5–ethynyl–2-deoxyuridine (EdU) cell proliferation assay, and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay. High glucose decreased the proportion of cardiac troponin T and α-actinin 2 positive mESCs as well as disrupted the α-actinin 2 striated pattern and the distribution of the cardiac myosin heavy chain α- and β isoforms. However, there was no alteration of the cellular EdU uptake nor the expression of the receptor of advanced glycation end-product (RAGE). High glucose also increased the presence of the oxidative stress marker nitrotyrosine as well as the number of TUNEL-stained nuclei in cardiaclike cells. Treatment with the antioxidant N-acetyl cysteine decreased the number of TUNEL-stained cells in high glucose and improved the α-actinin 2 striated pattern. Hyperglycaemia negatively impacted the expression and cellular organisation of cardiac myofilament proteins in mESC-derived cardiomyocytes through oxidative stress. The results add further insights into the pathophysiological mechanisms of cardiac contractile dysfunction in diabetic cardiac developmental disease.

This article indicates that Arab Libyan EFL learners are incompetent in oral communication. Despite spending years in learning English, they fail to employ that knowledge in real communicative situations. This might be the outcome of inadequate teaching approaches that are employed by Arab EFL teachers. Diaaba (2016) pointed out that, their overreliance on traditional approaches which emphasize extensive linguistic input rather than communicative output result in what Wolff (2010 cited in ibed, 2016 p. 338) calls 'mute English learners', who can read and write but cannot speak. The purpose of this study was to uncover challenges facing Arabic-speaking ESL students as well as teachers .13 participations are included in this article. Research questions focused on the needs of the Arab Libyan-speaking ESL students, the factors that influence their learning, and the problems the teachers face in supporting these students. Data sources included teacher and student interviews, classroom observations the results revealed that Arab Libyan learners face challenges in speaking skills due to linguistic and psychological barriers. The study concluded that several important challenges for teachers and students exist, including time, language support, and knowledge insufficient exposure to the target language and frequent use of Arabic were the main factors contributing to the Libyan EFL learners' speaking difficulties. Lack of speaking activities and overemphasis on accuracy at the expense of fluency by the teachers were other contributing factors.

Keywords: Speaking skills; speaking difficulties; instructional approaches; linguistic and psychological barriers

Abstract

Hyperglycaemia, a key metabolic abnormality in diabetes mellitus, is implicated in pathological cardiogenesis during embryological development. However, the underlying mechanisms and potential therapeutic targets remain unknown. We, therefore, studied the effect of hyperglycaemia on mouse embryonic stem cell (mESC) cardiac differentiation. The mESCs were differentiated via embryoid body (EB) formation and cultured under conditions with baseline (25 mM) or high (50 mM) glucose. Time-lapse microscopy images of pulsatile mESCs and Ca2+ntransients were recorded. Biomarkers of cellular changes were detected using immunocytochemistry, terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay, and Western blot analyses. Differentiated, spontaneously beating mESCs stained positive for cardiac troponin T, α-actinin 2,nmyosin heavy chain, and connexin 43. Hyperglycaemia decreased the EB diameter and number of beating EBs as well asnthe cellular amplitude of contraction, the Ca2+ transient, and the contractile response to caffeine (1 mM), but had no effect. on the expression of the sarco-endoplasmic reticulum calcium transport ATPase 2 (SERCA 2). Furthermore, hyperglycaemia decreased the expression of B cell lymphoma 2 (Bcl-2) and increased the expression of cytoplasmic cytochrome c and the number of TUNEL-positive cells, but had no effect on the expression of one of the mitochondrial fusion regulatory proteins, optic atrophy protein 1 (OPA1). Overall, hyperglycaemia suppressed the mESC cardiomyocyte-like differentiation and induced contractile dysfunction. The results are consistent with mechanisms involving abnormal Ca2+ handling and mitochondrial-dependent apoptosis, factors which represent potential therapeutic targets in developmental diabetic cardiac disease.