Background: Enlargement of inferior nasal turbinates is a common cause of nasal obstruction. There are many surgical methods used to treat hypertrophied inferior turbinates such as electrocautery, partial turbinectomy, microdebrider reduction and radiofrequency thermal ablation (RFTA). The mucosal condition after surgery is an important factor that should be considered in evaluation of different techniques. Objective: This study aimed to examine the microstructural appearance of the ciliated epithelial tissues of inferior turbinates by light microscope. It was done by cytological examination of inferior turbinate mucosa in patients treated by radiofrequency thermal ablation (RFTA) and bipolar electro cautery (BEC) for hypertrophied inferior turbinates. Methods: A prospective study performed on 36 patients with hypertrophied inferior turbinates causing nasal obstruction. Patients were divided into two groups: group (A) were managed with radiofrequency thermal ablation (RFTA) and group (B) were managed with bipolar electro cautery (BEC). Cytological examination of nasal mucosa was done 3 months postoperatively. Results: by cytological microscopic examination, 88% of cases of group (A) showed no abnormalities in ciliated epithelial cells. only 12% of cases showed abnormalities in the form of damage in the ciliated cells with an architectural rearrangement. In group (B) 78% of patients showed abnormalities in cilia and epithelial cell damage and only 22% of them showed no abnormalities. Conclusion: Radiofrequency thermal ablation (RFTA) seems to be a good modality dealing with the problem of inferior turbinate hypertrophy. It showed minimal injury to turbinate mucosal tissues with good ciliary structure, along with an intact and functioning epithelium

Enlargement of inferior nasal turbinates is a common cause of nasal obstruction. Surgical methods used to treat hypertrophied inferior turbinates such as electrocautery, cryosurgery, partial turbinectomy, laser turbinoplasty, microdebrider reduction and radiofrequency thermal ablation (RFTA).The mucosal condition after surgery is an important factor that should be considered in evaluation of different techniques. This study aimed to examine the microstructural appearance of the ciliated epithelial tissues by light microscope.It was done by cytological examination of inferior turbinate mucosain patients treated by radiofrequency thermal ablation (RFTA) and bipolar electrocautery (BEC) of hypertrophied inferior turbinates. A prospective study included thirty six patients with hypertrophied inferior turbinates causing nasal obstruction not responding to medical treatment. Patients were divided into two groups: group(A) were managed with radiofrequency thermal ablation (RFTA)and group (B)were managed with bipolar electrocautery (BEC).Routine nasal examination was done with the aids of anterior rhinoscopy and nasal endoscopy. The postoperative follow up period wasthree months. cytological examination of nasal mucosa was done postoperatively. Results: there was no abnormalities in ciliated epithelial cells and only 12% of cases showed abnormalities in the form of damage in the ciliated cellswith anarchitectural rearrangement.While, the majority of sample showed abnormalities in cilia and epithelial cell damage. 78% of patients had abnormal ciliated epithelial cells im group B. Radiofrequency thermal ablation seems to be a good modality dealing with the problem of inferior turbinate hypertrophy. It showed minimal injury to turbinatemucosal tissues with good ciliary structure, along with an intact and functioning epithelium.‏

Abstract: This study aims to analyzing demographic data of patients, isolate and identify Candida species, which causes vaginal infections, and a study of its prevalence among pregnant women in Sorman city, Libya.210 specimens collected from patients admitted to the Maternity Care Center in the combined clinic. carried immediately to the Microbiology Laboratory in the National Cancer Institute, Subrata, Libya for direct microscopy, culturing, and characterization. Each participant was given an interview questionnaire and asked about their age, educational level, employment position, and history of recurrent vaginal yeast infection. Chronic diseases were also listed on the data collecting form. Identification of Candida species using Chrome agar: A total of 100 isolates have been recovered in this study, of which 72isolates were obtained as pure cultures on Chrome agar medium. According to their color on Chrome agar, these 72 colonies were categorized to 5 main species namely Candida albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis. Genotypic identification of Candida species in this investigation was validated by the ITS tree. Eight strains from this investigation were found in the Candida albicans clade, which had a high bootstrap value of 99 percent ML/99 percent MP. These were therefore identified as Candida albicans., Within the Candida glabrata clade, three isolates were grouped together, demonstrating a strong support value of 99% ML/99%MP. These strains were recognized as belonging to the C. glabrata species, while one isolate was recognized as belonging to the C. tropicalis species, with a high support value of 99% ML/99%MP.

 Keywords: VVC infections, candidemia patients, genotypic identification, and Candida albicans

Abstract: The current study was aimed for isolation, identification and preservation of mycobiota associated with the olive oil processing wastes (Fatura) collected from different cities in Al-Gabal Al-Gharby, Libya, screening the cold-active lipolytic activity of the isolated fungi and selection of the highest cold-active lipase producers. 31 fungal species belong to 12 genera were isolated from these samples with total CFUs of 29560. Fusarium was the most common genus at total CFUs of 9020 and comprising 30.51% from all fungi, followed by Aspergillus, that recorded 25.44% from all fungi. Penicillium was ranked third, nine different species were present. A total of 100% of samples were found to have CFUs of 5140 and 17.4% of all fungi. On lipase production agar medium at two temperatures, 10 and 20°C, 102 fungal isolates from 31 species were tested for their lipolytic activity. The majority of fungi could produce lipase activity at 20°C, where 98 out of 102 isolates the highest lipase producers was higher at 10°C (25) than at 20°C (16). The most active isolates were Alternaria, Fusarium, and Penicillium. Molecular identification of the most active four isolates was carried out by sequencing their internal transcribed spacer region (ITS).

 Keyword: Olive oil, cold active enzymes, Lipase, fungi, lipolytic activity, Fatura.

 Food poisoning caused by bacterial agents is a worldwide problem, usually accompanied by unpleasant symptoms and may be sever leading to death. Natural compounds from marine algae namely flavonoids may play a role in the remedy of this condition. Aim: This research aims to assess the potency of flavonoids extracted from Enteromorpha intestinalis and Caulerpa prolifera as antibacterial agents. Methods: E. intestinalis was collected from Western Libyan Coast and C. prolifera was collected from Farwa Island. The antimicrobial activity and determination of minimum inhibitory concentration of algal flavonoid containing extracts was performed in vitro against some positive and negative Gram bacteria. Results: Crude extract containing flavonoids from E. intestinalis were more effective than C. prolifera extract against Staphylococcus aureus with antimicrobial essay (25-28+1 and 14.5-37.5+0.5-1.5), MIC (50 and 50-250 µg/mL), MBC (75 and 75-250 µg/mL). In Bacillus cereus, the antimicrobial assay (19-24.5+0.5-1.5: 24+1), MIC (50-250 + 100 µg/mL) and MBC (250 and 125 µg/mL). On the other hand, flavonoids containing extract from C. prolifera were more effective than E. intestinalis against Enterohemorrhagic Escherichia coli O157 EHEC O157 (25-28+1: 14-18.5+0.5-1.5), MIC (100-250:100-500 µg/mL) and MBC (150-250 and 250-500 µg/mL). Salmonella enterica qualitatively combat by flavonoid from E. intestinalis (13.5-14+0.5-1: 10.5-13.5+0.5-1.5), MIC (100-250: 250 µg/mL) and MBC (100-250: 250 µg/mL). Flavonoids from C. prolifera (4 strains: 2 strains) were effective against S. enterica. Crude flavonoids from both algae were not effective against Bacillus pumilus. Conclusion: Data from this study could conclude that flavonoid extracts from E. intestinalis and C. prolifera could be used against foodborne bacterial agents